Study Reveals Mechanisms Cancer Cells Use to Establish Metastatic Brain Tumors

6 03 2014

New research from Memorial Sloan Kettering provides fresh insight into the biologic mechanisms that individual cancer cells use to metastasize to the brain. Published in the February 27 issue of Cell, the study found that tumor cells that reach the brain — and successfully grow into new tumors — hug capillaries and express specific proteins that overcome the brain’s natural defense against metastatic invasion.

 Metastasis, the process that allows some cancer cells to break off from their tumor of origin and take root in a different tissue, is the most common reason people die from cancer. Metastatic brain tumors are ten times more common than primary brain cancers.

Yet most tumor cells die before they can take root in the brain, which is better protected than most organs against colonization by circulating tumor cells. To seed in the brain, a cancer cell must dislodge from its tumor of origin, enter the bloodstream, and cross densely packed blood vessels called the blood-brain barrier. Until now, little research has been done into how metastatic brain tumors develop, but previous mouse experiments that imaged metastatic breast cancer cells over time have shown that of those cancer cells that do make it to the brain, fewer than one in 1,000 survive.

“We didn’t know why so many of these cells die,” says Joan Massagué, PhD, Director of the Sloan Kettering Institute and senior author of the study. “What kills them? And how do occasional cells survive in this vulnerable state — sometimes hiding out in the brain for years — to eventually spawn new tumors? What keeps these rare cells alive and where do they hide?”

In the Cell study, Dr. Massagué, with Fellow Manuel Valiente, PhD, and other team members, found that in mouse models of breast and lung cancer — two tumor types that often spread to the brain — many cancer cells that enter the brain are killed by astrocytes. These killer cells, the most common type of brain cell, secrete a protein called Fas ligand.

When cancer cells encounter this protein, they are triggered to self-destruct. The exceptional cancer cells that escape the astrocytes do so by producing a protein called Serpin, which acts as a sort of antidote to the death signals fired at them by nearby astrocytes.

After imaging defiant metastatic cells in the brains of mice, researchers noticed that the cells that were able to survive grew on top of blood capillaries, each cell sticking closely to its vessel “like a panda bear hugging a tree trunk,” Dr. Massagué says. They found that the tumor cells produce a protein that acts like Velcro to attach the cells to the outer wall of a blood vessel.

“This hugging is clearly essential,” Dr. Massagué explains. “If a tumor cell detaches from its vessel, it gets killed by nearby astrocytes. By staying on, it gets nourished and protected, and may eventually start dividing to form a sheath around the vessel.”

Under the microscope, the researchers watched these sheaths of cancer cells around the blood capillaries grow into tiny balls, which eventually became tumors. “Once you’ve seen it, you can never forget this image,” Dr. Massagué says.

The tumor-cell survival factors uncovered by this study might one day be targeted with drugs to further diminish people’s risk of metastasis. Dr. Massagué is particularly interested in the ability of tumor cells to hug blood vessels, as he suspects this behavior may be essential for the survival of metastatic cancer cells not only in the brain but also in other parts of the body where metastatic tumor growth can occur.

“Most cancer patients are actually at risk of having their tumor spread to multiple sites,” Dr. Massagué notes. For example, breast cancers can metastasize to the bones, lungs, and liver, as well as to the brain. “What we may be looking at,” he adds, “is a future way to prevent metastasis to many organs simultaneously” using drugs that make tumor cells let go of the blood vessels they cling to.

 

This work was supported by NIH grants P01-CA129243 and U54-163167, DOD Innovator award W81XWH-12-0074, and the Alan and Sandra Gerry Metastasis Research Initiative.

 

 

Mskcc.org [en línea] New York (USA): mskcc.org, 6 de marzo de 2014 [ref. 27 de febrero de 2014] Disponible en Internet: http://www.mskcc.org/pressroom/press/study-reveals-mechanisms-cells-use-establish-metastatic-brain-tumors



Las células tumorales de colon que hacen metástasis en el hígado se vuelven más agresivas en respuesta al nuevo microambiente hostil

27 10 2011

Determinadas células sanas del hígado promueven una respuesta adaptativa en células de cáncer colorectal inhibiendo su proliferación y provocando su muerte. Las células tumorales que se adaptan, cambian su comportamiento y su morfología, favoreciendo la migración.

Investigadores del Instituto de Investigación Biomédica de Bellvitge (IDIBELL) y del Instituto Catalán de Oncología (ICO), coordinados por David García Molleví, han publicado los resultados de este estudio en la revista Neoplasia.

El microambiente celular (denominado estroma) tiene un papel dual en el control del desarrollo normal o maligno de las células: por un lado, impide el crecimiento de proliferación anormal (neoplasia) en los tejidos normales mientras que por el otro puede potenciar el crecimiento y la invasión tumoral en la progresión del cáncer.

El cáncer es un tejido complejo dónde actúan diferentes tipos de células (el 80% son de un tipo denominado fibroblastos) que conviven con otras células creando un microambiente particular. El balance entre estas poblaciones: el ambiente, puede determinar el destino del tumor.

Un microambiente hostil

El objetivo del estudio es comparar la influencia del microambiente en tres situaciones diversas: en un tejido colorectal sano, en un tumor colorectal primario y en un tumor colorectal que ha hecho metástasis al hígado.

En el tumor primario, los fibroblastos del colon aumentan la proliferación de las células malignas y las protegen de la muerte celular programada (apoptosis). En cambio, en la situación de metástasis al hígado, las células tumorales se encuentran en un ambiente hostil de fibroblastos hepáticos, no sólo disminuye su proliferación, sino que muchas mueren. Las células que consiguen adaptarse a este ambiente cambian su comportamiento y su morfología para favorecer su migración. Estas células tumorales adaptadas son más agresivas.

Según David Garcia Molleví, coordinador del estudio, este descubrimiento puede ser útil en la práctica clínica ya qué “abre las puertas a estudiar el mecanismo o los factores de los fibroblastos hepáticos que producen la muerte de las células tumorales colorectales para utilizarlos en el futuro como herramientas terapéuticas contra el tumor de colon metastático”.

Cambios en las células tumorales de colon que hacen metástasis en el hígado

Referencia del artículo

 Mireia Berdiel-Acer, Monika Bohem, Adriana López-Doriga, August Vidal, Ramon Salazar, Maria Martínez-Iniesta, Cristina Santos, Xavier Sanjuan, Alberto Villanueva and David G. Molleví. Hepatic Carcinoma-associated fibroblasts promote an adaptative response in colorectal cancer cells that inhibit proliferation and apoptosis. Neoplasia. Volume 13 Number 10 October 2011 pp. 931946